Evaluation of performance of serological tests
by Nicholas Ruuri, Kiriinya
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This book describes Evaluation of performance of serological tests for the detection of Foot and Mouth Disease virus antibodies in Kilifi County, Kenya. Objective: To determine the sensitivity of assays Anigen © FMD NSP Ab ELISA -Korea, NSP Priocheck® kits with Liquid Phase Blocking Enzyme Linked Immunosorbent Assay compared with Virus neutralization test ( the gold standard) and Identification of prevalent serotypes of foot and mouth disease virus in Kilifi County coast of Kenya 2014. Design: cross-sectional study design. Setting of the study: Kilifi County. Subjects: 421 cattle serum. Results: The results showed that both Anigen © FMD NSP Ab ELISA (Korea), NSP Priocheck® Netherlands assays had low sensitivity but high specificity. Liquid Phase Blocking ELI SA had the highest sensitivity 100%. LPBE had a Positive Predictive Value (PPV) =100%, Anigen © FMD NSP Ab ELISA-Korea 94.44% and NSP Priocheck® Netherlands had the lowest 44.74% compared with the VNT 100%. The Cohen's kappa coefficient showed a perfect agreement level of 90%, 93%, 100 and 100% respectively. Conclusion: This study showed no significant difference in sensitivity and both structural and non-structural tests.
Nicholas Ruuri, Kiriinya
Nicholas K. Ruuri, has over 10 years experience in lab work & data analysis. I hold a masters degree in medical virology from INSTITUTE OF TROPICAL MEDICINE AND INFECTIOUS DISEASES, jomo Kenyatta university, BSc. medical lab science and Bsc. applied biology(micro opt). Currently working at Alupe University college Kenya as a lab technologist.
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LAP Lambert Academic Publishing
0.22 x 0.15 x 0.005 m; 0.131 kg