Protein Scaffolds - Design, Synthesis, and Applications

by Springer US
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Springer US Protein Scaffolds - Design, Synthesis, and Applications
Springer US - Protein Scaffolds - Design, Synthesis, and Applications

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This volume provides a wide range of methods and protocols detailing various protein structures as platforms for building architectures with targeted application. Chapters guide the readers through exploiting a number of protein scaffolds including virus nanoparticles, elastin and collagen peptides and proteins, and other protein templates for either building materials or presentation of ligands. Site-specific bioconjugation methods, some unique protein architectures, and techniques that exploit peptide amphiphile micelles and assembly of chaperones are also featured. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Protein Scaffolds: Design, Synthesis, and Applications aims to ensure successful results in the study of this vital field.

Further information

Illustrations Note:
24 SW-Abb., 52 Farbabb.
Table of Contents:

1. Nanoreactors via Encapsulation of Catalytic Gold Nanoparticles within Cowpea Chlorotic Mottle Virus

Aijie Liu, Mark V. de Ruiter, Stan J. Maassen, and Jeroen J.L.M. Cornelissen


2. Encapsulation of Active Enzymes within Bacteriophage P22 virus-like Particles

Dustin P. Patterson


3. Encapsulation of Exogenous Proteins in Vault Nanoparticles

Meng Wang, Danny Abad, Valerie A. Kickhoefer, Leonard H. Rome, and Shaily Mahendra


4. Enzyme Encapsulation in an Engineered Lumazine Synthase Protein Cage

Yusuke Azuma and Donald Hilvert


5. Metal Ion-induced Self-Assembly and Packaging of CCMV Nanocapsules

Lise Schoonen, Mark B. van Eldijk, and Jan C. M. van Hest


6. Modification of CCMV Nanocages for Enzyme Encapsulation

Lise Schoonen and Jan C. M. van Hest


7. Synthesis of Cross-linked 2-aminoethyl Methacrylate in P22 Viral Capsid via Atom-transfer Radical Polymerization

Shefah Qazi   


8. Synthesis of Metal-organic Frameworks on Tobacco Mosaic Virus Templates

Shaobo Li and Jeremiah J. Gassensmith


9. TMV Disk Scaffolds for Making sub-30 nm Silver Nanorings

Serene Bayram, Omar Zahr, Julia Del Re, and Amy Szuchmacher Blum


10. TMV Disk Scaffolds for Making sub-30 nm Silver Nanorings

Serene Bayram, Omar Zahr, Julia Del Re, and Amy Szuchmacher Blum


11. Synthesis of Triangular Silver and Gold Nanoprisms Using Consensus Sequence Tetratricopeptide Repeat Proteins

Xi Geng  and Tijana Z. Grove


12. Type III Secretion Filaments as Templates for Metallic Nanostructure Synthesis

Anum Azam Glasgow


13. Incorporation of Non-canonical Amino Acids into Proteins by Global Reassignment of Sense Codons

Katharine Y. Fang, Seth A. Lieblich, and David A. Tirrell


14. Modification of Protein Scaffolds via Copper-catalyzed Azide‚ÄďAlkyne Cycloaddition

Stanislav Presolski


15. Antibody Modification of p-aminophenylalanine-containing Proteins

Adel M. ElSohly, Chawita Netirojjanakul, and Matthew B. Francis


16. Cross-linked Collagen Gels using Gold Nanoparticles

Katarzyna Slowinska


17. Genipin Cross-linking Of Elastin and Elastin-based Proteins

Lisa D. Muiznieks


18. Characterization of Self-Assembled Protein Scaffolds from Collagen-mimetic Peptides

Devyani Shukla, Jayeeta Lahiri, and Avanish Singh Parmar

  19. Design and Characterization of Fibers and Bionanocomposites Using the Coiled-coil Domain of Cartilage Oligomeric Matrix Protein

Priya Katyal and Jin Kim Montclare


20. Preparing Genes for Repetitive Elastin-like Polypeptides Using Gibson Assembly

James K. Deyling, Edward M. Turk, and Nolan B. Holland


21. Peptide Amphiphile Micelles for Vaccine Delivery

John C. Barrett and Matthew V. Tirrell


22. Controlled Assembly of the Filamentous Chaperone Gamma-prefoldin into Defined Nanostructures

Douglas S. Clark and Dominic J. Glover


23. Advances in the Application of Designed Ankyrin Repeat Proteins (Darpins) As Research Tools and Protein Therapeutics

Ykelien L. Boersma


Udit, Andrew K.;Udit
Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Media Type:
Springer US
1st ed. 2018
Number of Pages:

Master Data

Product Type:
Hardback book
Release date:
June 5, 2018
Package Dimensions:
0.254 x 0.178 x 0.021 m; 0.822 kg
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