Corneal Regeneration - Methods and Protocols

by Springer US
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Springer US Corneal Regeneration - Methods and Protocols
Springer US - Corneal Regeneration - Methods and Protocols

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Description

This volume details different laboratory techniques used to develop regenerative therapies that help treat corneal blindness. Chapters guide readers through methods and protocols on optimal cell culture conditions, gene-editing techniques, multiple types of scaffold for corneal regeneration, how to evaluate the success of these therapies, and cell and material characterization techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Corneal Regeneration: Methods and Protocols aims to be useful for new and experienced laboratory researchers working on different aspects of corneal regeneration.

Further information

Illustrations Note:
XII, 250 p. 66 illus., 43 illus. in color.
Language:
English
Table of Contents:

1. Isolation and Culture of Corneal Stromal Stem Cells



Richard M Nagymihaly, Morten C Moe, and Goran Petrovski



2. In vitro Expansion of Corneal Endothelial Cells for Transplantation



Kim Santerre, Isabelle Xu, Mathieu Thériault, and Stéphanie Proulx



 



3. Primary Culture of Cornea-Limbal Epithelial Cells In Vitro



Finbarr O'Sullivan 



 



4. Optimization of Human Limbal Stem Cell Culture by Replating a Single Limbal Explant



Marina López-Paniagua, Teresa Nieto-MiguelGalindo, Laura García-Posadas, Ana de la Mata, Rosa M. Corrales, Margarita Calonge, and Yolanda Diebold



 



5. A Guide to the Development of Human Cornea Organoids from Induced Pluripotent Stem Cells in Culture



James W Foster, Karl Wahlin, and Shukti Chakravarti




 



6. Gene Editing for Corneal Stromal Regeneration



Tara Moore, Connie Chao-Shern , Larry DeDionisio, Katie Christie, and Andrew M.Nesbit



 



7. Preparation and Administration of Adeno-associated Virus Vectors for Corneal Gene Delivery



Liujiang Song, Jacquelyn J. Bower, and Matthew L. Hirsch




 



8. The Self-assembly Approach as a Tool for the Tissue Engineering of a Bi-lamellar Human Cornea



Gaëtan Le-Bel, Pascale Desjardins, Camille Couture, Lucie Germain, and Sylvain L. Guérin



 



9. Formation of Corneal Stromal-like Assemblies using Human Corneal Fibroblasts and Macromolecular Crowding



Mehmet Gürdal, Gülinnaz Ercan, and Dimitrios I. Zeugolis



 



10. Preparation of Dried Amniotic Membrane for Corneal Repair



Andrew Hopkinson , Emily R Britchford, and Laura E Sidney



 



11. Fabrication of Corneal Extracellular Matrix Derived Hydrogels



Mark Ahearne and Julia Fernández-Pérez



 



12. Synthesis and Application of Collagens for Assembling a Corneal Implant



Elle Edin, Fiona Simpson, and May Griffith



 



13. Development and Validation of a 3D in vitro Model to Study the Chemotactic Behaviour of Corneal Stromal Fibroblasts



Evrim Ceren Kabak, Julia Fernández-Pérez, and Mark Ahearne




 



14. Femtosecond Laser Assisted Surgery for Implantation of Bioengineered Corneal Stroma to Promote Corneal Regeneration



Neil Lagali and Mehrdad Rafat




 



15. The use of Animal Models to Assess Engineered Corneal Tissue



Robert Thomas Brady and Peter W Madden



 



16. X-ray Diffraction Imaging of Corneal Ultrastructure



Keith M Meek, Andrew J. Quantock, Sally Hayes, and James Bell

Edition:
1st ed. 2020
Number of Pages:
250
Editor:
Ahearne, Mark;Ahearne
Remarks:
Includes cutting-edge methods and protocols


Provides step-by-step detail essential for reproducible results


Contains key notes and implementation advice from the experts

Media Type:
Hardcover
Publisher:
Springer US

Master Data

Product Type:
Paperback book
Package Dimensions:
0.259 x 0.196 x 0.018 m; 0.699 kg
GTIN:
09781071605981
DUIN:
1BHLDNMTILG
$161.66
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