Monoclonal antibodies anti-beta2-glycoprotein I - Production, characterization, analytical applications

by Stella, Carolina Nigro
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Stella, Carolina Nigro Monoclonal antibodies anti-beta2-glycoprotein I - Production, characterization, analytical applications
Stella, Carolina Nigro - Monoclonal antibodies anti-beta2-glycoprotein I - Production, characterization, analytical applications

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Description

Beta2-glycoprotein I ( 2GPI) detection is important for the accurate diagnosis of autoimmune and chronic inflammatory disease. This book describes the main steps towards a successful obtaining, initial characterization, analytical applications and lab-scale production of a set of anti- 2GPI monoclonal antibodies. The 2GPI antigen purification was comprehensively explored and six different hybridomas were obtained after cell fusion of immunized mouse lymphocytes with SP2-O murine myeloma cells. The antibodies were purified after hybridomas expansion from ascites (in vivo) and conventional cell culture (in vitro) and their reactivity was evaluated towards the 2GPI antigen either adsorbed to solid phase support (Dot-Blot, immunoblotting, direct ELISA), or in solution (indirect ELISA). 2GPI recognition was achieved with one of the clones in paraffin embedded tissues (immunohistochemistry), and in blood and lymph (ELISA). The application of 2GPI recognition to a sepsis and bacterial translocation animal model granted an unexpected and exciting sequence of achievements concerning the pathophysiology of autoimmunity control by this protein.

Contributors

Author:
Stella, Carolina Nigro
Ferreira Gomes, Lígia

Further information

Biography Artist:
MSc in Pharmaceutical Sciences (Clinical Chemistry) from University of São Paulo (USP), cytologyst and faculty member at Faculdades Oswaldo Cruz, Centro Universitário São Camilo and Universidade Paulista. Lígia Ferreira Gomes is PhD in Biochemistry and tutored this work at Faculty of Pharmaceutical Sciences - USP, Brasil
Language:
Englisch
Number of Pages:
92
Media Type:
Taschenbuch
Publisher:
LAP Lambert Academic Publishing

Master Data

Product Type:
Paperback book
Release date:
May 13, 2013
Package Dimensions:
0.22 x 0.15 x 0.005 m; 0.186 kg
GTIN:
09783659393853
DUIN:
11BFDJD3RF9
$38.99
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