Cloning, Expression and Characterization of Cyt2Aa1 Toxin

by Abdel Rahman, Mohamed
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Abdel Rahman, Mohamed Cloning, Expression and Characterization of Cyt2Aa1 Toxin
Abdel Rahman, Mohamed - Cloning, Expression and Characterization of Cyt2Aa1 Toxin

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Description

A reliable system for expression and purification of the recombinant Cyt2Aa1 toxin has been developed. The recombinant Cyt2Aa1 toxin has been produced, characterized, followed by the construction of the cysteine mutants V186C and L189C by site directed mutagenesis.The hemolytic activity of the V186C mutant exceeds that of wild type Cyt2Aa1 toxin and of the L189C mutant.Calcein release assay experiments have been done to examine the activity of the toxin with different artificial liposomes. It was found that Cyt2Aa1 toxin is very active with DMPC, DMPC+DMPG unilamellar liposomes.

Contributors

Author:
Abdel Rahman, Mohamed
Palmer, Michael

Further information

Biography Artist:
Mohamed Abdel Rahman received his B.S. degree in pharmacy from Al-Azhar University in Egypt in 2001. He participated as a teaching assistant and a researcher at Biology Department in Waterloo University in Canada. In 2010 he received his MSc. in Biology from Waterloo University and continued to study for his Ph.D. from the same university to date.
Language:
Englisch
Number of Pages:
80
Media Type:
Taschenbuch
Publisher:
LAP Lambert Academic Publishing

Master Data

Product Type:
Paperback book
Release date:
December 13, 2012
Package Dimensions:
0.22 x 0.15 x 0.005 m; 0.168 kg
GTIN:
09783844326321
DUIN:
KVE6UUIRCII
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